Instructions
1. What you need
- A target amino-acid sequence (the protein you want an aptamer to bind).
- Optionally, custom DNA prefix and suffix strings if you want to override the built-in primers.
2. Choose parameters
- Variant length: length (in bases) of the variable region generated between the prefix and suffix. Larger values mean a much larger search space.
- Total length: the full aptamer length (prefix + variant + suffix). Must be at least variant length + 2.
- Max sequences: upper bound on how many candidates the search will consider.
3. Submit and wait
- Click Submit. The job is queued in the background and you are redirected to a live progress view.
- The progress bar and remaining-time estimate update every second or so.
- You can bookmark the result URL — it stays valid for 90 days.
4. Read the results
- The top 25 candidates are shown, ranked by interaction probability (higher = more likely to bind the target).
- Each row shows the candidate sequence, its predicted secondary structure (fornac visualization), and its MFE in kcal/mol.
- Use the Start New Task button to reset the form and submit a different query.